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Characterization of the lectin binding pattern in human spermatozoa after swim-up selection

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dc.contributor.authorGómez-Torres, María José
dc.contributor.authorAvilés, Manuel
dc.contributor.authorGirela, Jose Luis
dc.contributor.authorMurcia, Verónica
dc.contributor.authorFernández-Colom, Pedro José
dc.contributor.authorRomeu, Alberto
dc.contributor.authorJuan, Joaquín de
dc.date.accessioned2017-11-09T15:35:14Z
dc.date.available2017-11-09T15:35:14Z
dc.date.issued2012
dc.description.abstractCapacitation is characterized by a hyperactivated pattern of sperm motility. The acquisition of highly motility is present in the early stages of capacitation. Sperm progressive motility is one of the most important parameters for determining the suitability of semen for processing. However, previous studies have shown that some sperm showing good motility have membrane damage. The aim of our study was to characterize the lectin staining pattern on the sperm plasma membrane of unselected and selected human sperm of normozoospermic donors. Sperm selection was performed by the swim-up technique. Fourteen samples from healthy consenting donors classified as normozoospermic according to the World Health Organization were used. We observed changes in the distribution of the carbohydrate residues after the swim-up selection. With Triticum vulgaris, the most abundant pattern was dotted labeling all over the head plasma membrane in the unselected sperm. However, this lectin was distributed homogenously over the acrosomal region after selection. With Arachis hypogaea, the most abundant pattern in fresh sperm was a highly stained acrosomal region. In the highly motility sperm population, the most frequent pattern was dotted fluorescence on the acrosomal region and a highly stained equatorial segment. Meanwhile, with the Aleuria aurantia and Canavalia ensiformis lectins, the most representative patterns were the same before and after the swim-up selection. Our data indicate that modifications which occur in carbohydrate residues during swim-up selection could be important for the regulation of progressive motility and prepare the sperm for capacitation.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.eisbnHistology and histopathology, Vol. 27, n.º 12 (2012)es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/54471
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectGlycocalyxes
dc.subjectCarbohydratees
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleCharacterization of the lectin binding pattern in human spermatozoa after swim-up selectiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.27, nº12 (2012)