Publication: Use of interphase cytogenetics in demonstrating specific chromosomal aberrations in solid tumors new insights in the pathogenesis of malignant
melanoma and head and neck squamous cell carcinoma
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Date
2000
Authors
Poetsch, M. ; Dittberner, T. ; Woenckhaus, C. ; Kleist, B.
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Publisher
Murcia : F. Hernández
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Description
Abstract
The detection of structural and numerical
chromosomal aberrations is an important part of the
characterization of tumors and genetic diseases. The
direct demonstration of DNA sequences in interphase
nuclei and metaphases by fluorescence in situ
hybridization (FISH) has been termed interphase
cytogenetics. It has been proven as a powerful technique
to detect specific aberrations in a wide variety of cell
types, including paraffin-embedded tissue. Nowadays a
standard method in leukemia and lymphoma, interphase
cytogenetics contributes mainly to the diagnosis in these
tumors and helps to classify soft tissue tumors.
Therefore FISH is mandatory for the choice of therapy
in these tumors. In contrast to the aforementioned, up to
now, the value of FISH in solid tumors is mostly limited
to pure research and contributes in this way to our
understanding of tumor biology. But with the use of
paraffin-embedded tissue and the first results obtained, it
seems very likely that a direct correlation between
histological classification and cytogenetic characteristics
of solid tumors can be achieved in the near future. This
information might not only provide insights into tumor
biology, but could also contribute to a different tumor
classification, a sort of risk estimation, where we might
predict the possible biological behavior of solid tumors.
This could greatly influence further therapeutic decisions
thus establishing the FISH technique as an indisputable
part in the diagnosis of solid tumors.
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