Publication: Brain CYP1A in seabream Sparus aurata exposed to Benzo(a)pyrene
Authors
Ortiz-Delgado, J.B. ; Segner, H. ; Sarasquete, C.
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Publisher
Murcia : F. Hernández
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Description
Abstract
This study compares basal and induced
expression of cytochrome P4501A-CYP1A in the brain
of gilthead seabream, Sparus aurata. Larval or adult
seabream were exposed to benzo(a)pyrene -B(a)P- and
the CYP1A response was assessed by analyzing CYP1A
mRNA (RT-PCR), CYP1A protein (expression levels:
ELISA, western blotting; cellular localization:
immunohistochemistry), and CYP1A catalytic activity
(7-ethoxyresorufin-O-deethylase-EROD).
In the brain of adult S. aurata, CYP1A
immunostaining was generally detected in the
vasculature. It was present in the neuronal fibers and
glial cells of the olfactory bulbs and the ventral
telencephalon. ELISA and RT-PCR analyses confirmed
CYP1A expression in the brains of non-exposed
seabream. B(a)P exposure led to increased CYP1A
staining mainly in neuronal fibers and glial cells of the
olfactory bulbs, but also in the vascular endothelia.
EROD activity, however, could not be detected in the
brain of adult seabream, neither in control nor in
exposed fish.
In the developing brain of S. aurata larvae,
immunohistochemical staining detected CYP1A protein
exclusively in endothelia of the olfactory placode and in
retina. Staining intensity of CYP1A slightly increases
with larval development, especially in vascular brain
endothelia. Exposing the larvae to 0.3 or 0.5 μg B(a)P/L
from hatching until 15 days post hatching (dph) did not result in enhanced CYP1A immunostaining in the brain.
In samples of whole seabream larvae, both from controls
and BaP treatments, neither CYP1A mRNA, protein nor catalytic activity were detectable.
The results demonstrate that CYP1A is expressed
already and inducible in the larval brain, but that the
regional and cellular expression differs partly between
larval and adult brain. This may have implications for
the toxicity of CYP1A-inducing xenobiotics on early
and mature life stages of seabream.
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