Publication: The appearance of myofibroblasts and
the disappearance of CD34-positive stromal
cells in the area adjacent to xanthogranulomatous
foci of chronic cholecystitis
Authors
Kuroda, Naoto ; Guo, L. ; Miyazaki, E. ; Hamauzu, T. ; Toi, M. ; Hiroi, Makoto ; Enzan, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We investigated the distribution of
myofibroblasts and CD34-positive stromal cells in
normal gallbladder and its pathological conditions
(cholecystitis, n=25) using immunohistochemistry and in
situ hybridization. In the wall of normal gallbladder,
myofibroblasts were generally absent from all layers, but
many CD34-positive stromal cells were observed in the
connective tissue layer. In chronic cholecystitis with
mild perimuscular fibrosis, a small to moderate number
of myofibroblasts appeared in the mucosal layer. In
chronic cholecystitis with marked perimuscular fibrosis,
a small to large number of myofibroblasts appeared
predominantly in the connective tissue layer, whereas
the number of CD34-positive stromal cells decreased at
the same location, although the number of
myofibroblasts increased. In chronic cholecystitis with
xanthogranulomatous foci, a small to large number of
myofibroblasts were observed in the periphery of the
xanthogranulomatous reaction and adjacent area. In
contrast, CD34-positive stromal cells were completely
absent or were limited to the area just around the
xanthogranulomatous reaction. Induction of collagen
type I and III mRNA was predominantly observed in the
cytoplasm of myofibroblasts associated with the marked
fibrosis, which consisted primarily of mature collagen
fibers, and in the cytoplasm of myofibroblasts around
the xanthogranulomatous reaction, respectively. Finally,
myofibroblasts were observed in all subtypes. The
increased number of myofibroblasts was most prominent
in the connective tissue layer of chronic cholecystitis
with marked perimuscular fibrosis or in the area adjacent
to xanthogranulomatous foci of chronic cholecystitis.
Under these conditions, CD34-positive stromal cells
tended to disappear from the connective tissue layer,
which exhibited an increase in myofibroblasts.
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