Publication: Paracervical ganglion in the female pig during prenatal development: morphology and immunohistochemical characteristics
Authors
Franke-Radowiecka, Amelia
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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Description
Abstract
The present study investigated the
development of the paracervical ganglion in 5-, 7- and
10-week-old porcine foetuses using double labelling
immunofluorescence method. In 5-week-old foetuses
single PGP-positive perikarya were visible only along
the mesonephric ducts. They contained DβH or VAChT,
and nerve fibres usually were PGP/VAChT-positive. The
perikarya were mainly oval. In 7-week-old foetuses, a
compact group of PGP-positive neurons (3144±213) was
visible on both sides and externally to the uterovaginal
canal mesenchyme of paramesonephric ducts. Nerve cell
bodies contained only DβH (36.40±1.63%) or VAChT
(17.31±1.13%). In the 10-week-old foetuses, the
compact group of PGP-positive neurons divided into
several large and many small clusters of nerve cells and
also became more expanded along the whole
uterovaginal canal mesenchyme reaching the initial part
of the uterine canal of the paramesonephric duct. The
number of neurons located in these neuronal structures
increased to 4121±259. Immunohistochemistry revealed
that PGP-positive nerve cell bodies contained DβH
(40.26±0,73%) and VAChT (30.73±1.34%) and were
also immunoreactive for NPY (33.24±1,27%), SOM
(23.6±0,44%) or VIP (22.9±1,13%). Other substances
studied (GAL, NOS, CGRP, SP) were not determined at
this stage of the development. In this study, for the first
time, the morphology of PCG formation in the porcine
foetus has been described in three stages of
development. Dynamic changes in the number of
neurons and their sizes were also noted, as well as the
changes in immunochistochemical coding of maturing
neurons.
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