Publication: MiR-129-5p/TRIP13 affects malignant phenotypes of colorectal cancer cells
Authors
Cao, Yongqing ; Huang, Fang ; Liu, Jiheng ; Qi, Hui ; Xiao, Jinjun
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/ 10.14670/HH-18-455
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info:eu-repo/semantics/article
Description
Abstract
Objective. Aberrant miR-129-5p expression
is a key modulator of cancer development. But how the
miRNA affects colorectal cancer (CRC) remains unclear.
This study was designed to illustrate the underlying
mechanism of miR-129-5p in CRC.
Methods. MiR-129-5p expression at cellular level
was assayed by qRT-PCR. Its role in CRC cell
phenotypes was studied by cell function experiments.
The binding relationship between miR-129-5p and
TRIP13 was analyzed and verified by bioinformatics
prediction and dual-luciferase detection. Furthermore,
the functional mechanism based on miR-129-5p and
TRIP13 in CRC was studied through rescue
experiments.
Results. CRC cell lines presented prominently lower
miR-129-5p levels than the normal colon epithelial cell
line. The forced miR-129-5p level suppressed CRC cell
growth. TRIP13 was proved to be a target of miR-129-
5p in CRC cells, and miR-129-5p overexpression
reduced TRIP13 expression. TRIP13 knockdown
resulted in cell cycle arrest. Additionally, TRIP13
overexpression restored the impacts of miR-129-5p
overexpression on cell malignant phenotypes and cell
cycle.
Conclusion. MiR-129-5p down-regulated TRIP13
expression, thereby restraining the malignant
progression of CRC cells. The findings may offer a new
target for molecular therapy of CRC.
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