Publication: A comparative study of extracellular matrix remodeling in two murine models of emphysema
Authors
Lopes, F.D.T.Q.S. ; Toledo, A.C. ; Olivo, C.R. ; Prado, C.M. ; Leick, E.A. ; Medeiros, M.C. ; Santos, A.B.G. ; Garippo, A. ; Martins, M.A. ; Mauad, T.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A single instillation of porcine pancreatic
elastase (PPE) results in significant airspace enlargement
on the 28th day after instillation, whereas cigarette
smoke (CS) exposure requires 6 months to produce mild
emphysema in rodents. Considering that there are
differences in the pathogenesis of parenchymal
destruction in these different experimental models, it is
likely that there may be different patterns of extracellular
matrix (ECM) remodeling. To evaluate ECM
remodeling, C57BL/6 mice were submitted to either a
nasal drop of PPE (PPE 28 Days) or exposed for 6
months to cigarette smoke (CS 6 months). Control
groups received either an intranasal instillation of saline
solution (Saline 28 Days) or remained without any
smoke inhalation for six months (Control 6 months). We
measured the mean linear intercept and the volume
proportion of collagen type I, collagen type III, elastin
and fibrillin. We used emission-scanning confocal
microscopy to verify the fiber distribution. Both models
induced increased mean linear intercept in relation to the
respective controls, being larger in the elastase model in
relation to the CS model. In the CS model, emphysema
was associated with an increase in the volume proportion
of fibrillin, whereas in the PPE model there was an
increase in the parenchymal elastin content. In both
models, there was an increase in collagen type III, which
was higher in the CS-exposed mice. We concluded that
ECM remodeling is different in the two most used
experimental models of emphysema.
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