Publication: Practical applications of some antibodies labelling the human retina
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Date
1993
Authors
Chevez, P. ; Font, R.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Eight enucleated eyes were promptly fixed in
10% formalin and processed for light microscopy.
Employing the ABC technique, we used antibodies
against neuron-specific enolase (NSE), synaptophysin
(SY), neurofilament (NF), glial fibrillary acidic protein
(GFAP), keratins, LEU-7 and S-100 protein. Using NSE
we found immunoreactivity in all sensory layers except
the outer segments of photoreceptors which were nonreactive.
SY showed a striking immunoreactivity of the
synaptic regions (outer plexiform and inner plexiform
layers). NF labelled the axons of the ganglion cells.
GFAP stained perivascular glial cells and Muller cells
only in areas of peripheral microcystoid degeneration.
Keratin was non-reactive in all layers. LEU-7 showed
that all layers were moderately reactive while the Muller
cell processes and the outer limiting membrane were
strongly immunoreactive. S-100 protein stained the
perivascular glial cells in the nerve fiber layer. We
conclude that NSE and LEU-7 are helpful markers for
most layers of the sensory retina; GFAP and LEU-7
clearly outline Miiller cells and GFAP immunoreacts
with astrocytes; NF is specific for axons and SY labels
vividly the synaptic regions.
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