Publication: Brucelosis bovina: estudio comparativo entre la electroforesis y la serología clásica
Authors
Bordallo Álvarez, L. ; Cuello Gijón, Francisco ; Crespo León, Fernando ; Gallego Ruiz, María del Carmen
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Facultad de Veterinaria
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Publisher
Murcia: Universidad de Murcia, Servicio de Publicaciones
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Se ha evaluado la electroforesis como técnica diagnóstica en bmceiosis bovina frente a tres praebas clásicas
en serología: seroaglutinación lenta de Wnght, seroaglutinación con 2-mercaptoetanol y reacción de fijación del
complemento.
Las fracciones proteínas totales. alfa- l . beta-2 y globulinas totales alcanzan valores significativamente más
elevados en los individuos con diagnóstico positivo por SAL o ME, mientras que los diagnosticados como
positivos por RFC muestran esta significación en los niveles de proteínas totales. albúmina, alfa-1 y beta-2
globulinas.
El análisis de datos centrados no diferencia entre individuos positivos o negativos en base a los resultados del
análisis electroforético. El análisis discriminante, en base a la cuantificación de las fracciones del proteinograma,
nos muestra la no concordancia entre los resultados positivos sospechosos y negativos de las pmebas cláf amente
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An evaluation of an electrophoretic analysis was carned out to estimate the value of this method as a diagnostic iechnic versus the classical ones: semm agglutination (SAT). 2-mercaptoethanol (ME)and complement fixation test (CFT). Serum fractions alfa-l. beta-2 with total proteins and total globulins reach amounts signifícantly higher in individuals with bnicellosis detected through ME or SAT. ln CFT. albumin, alfa-l and beta-2 globulins are the significative fractions. The centered data analysis shows no differentiation between positive individuals according to the results of the electrophoretic method. Through the discriminating analysis, it is noticed that the percentages of individuals detected as negative, suspicious or positive by means of the electrophoretic method are not coincident with those reported by each of the serological tests. Keywords: Brucellosis. Electrophoresis. Agglutination. Mercaptoethanol. Complement fixation test.
An evaluation of an electrophoretic analysis was carned out to estimate the value of this method as a diagnostic iechnic versus the classical ones: semm agglutination (SAT). 2-mercaptoethanol (ME)and complement fixation test (CFT). Serum fractions alfa-l. beta-2 with total proteins and total globulins reach amounts signifícantly higher in individuals with bnicellosis detected through ME or SAT. ln CFT. albumin, alfa-l and beta-2 globulins are the significative fractions. The centered data analysis shows no differentiation between positive individuals according to the results of the electrophoretic method. Through the discriminating analysis, it is noticed that the percentages of individuals detected as negative, suspicious or positive by means of the electrophoretic method are not coincident with those reported by each of the serological tests. Keywords: Brucellosis. Electrophoresis. Agglutination. Mercaptoethanol. Complement fixation test.
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