Publication: Anti-apoptotic activity in deep pelvic endometriosis
Authors
Abdalla Ribeiro, Helizabet S. ; Galvão, Maria Antonieta Longo ; Aoki, Tsutomu ; Aldrighi, José Mendes ; Ribeiro, Paulo Ayroza
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Since endometriosis is a proliferative disease
we evaluated the presence of anti-apoptotic factor (Bcl2)
and pro-apoptotic factor (Bax) in deep pelvic
endometriosis. A Cross-sectional observational study
was performed at Santa Casa de Misericórdia de São
Paulo, São Paulo, Brazil. Forty women aged 26 to 46
years with deep endometriosis were selected. They had
not been clinically treated for at least 3 months prior to
surgery and then underwent surgical laparoscopy to treat
the disease. During the surgery, tissue was collected
from the uterosacral ligaments and the rectosigmoid; an
endometrial biopsy was also performed as a control. All
interventions were performed by the same surgeon. The
specimens were sent for pathological and immunohistochemical
analyses; endometriosis was confirmed in all
patients. After the immunohistochemical reaction a
semi-quantitative evaluation of the staining intensity
(relative optical density-ROD) was conducted, applying
the digital densitometric analysis system. In the
uterosacral ligaments 97.5% of the specimens were
positive for Bcl2 whereas in the rectosigmoid 100%
were positive. In the endometrium we observed that
87.5% were positive for Bcl2. BAX expression was null
in the rectosigmoid and in the endometrium. In the
uterosacral ligaments 2.5% of the specimens expressed
BAX. The relative optical density of Bcl2 was higher in
the rectosigmoid and in the uterosacral ligament when
compared to the endometrium, 0.141±0.002;
0.129±0.001, respectively (p<0.01). We concluded that
the anti-apoptotic factor Bcl-2 was expressed in all
studied specimens, but in a higher staining intensity in
the rectosigmoid and in the uterossacral ligaments in
comparison to the endometrium. The pro-apoptotic
factor Bax had virtually no expression in the studied
tissues.
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