Publication: Histological and biochemical characteristics of the rabbit anterior cruciate ligament in comparison to potential autografts
Authors
Hoyer, Mariann ; Meier, Carola ; Kohl, Benjamin ; Lohan, Anke ; Kokozidou, Maria ; Schulze Tanzil, Gundula
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-723
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info:eu-repo/semantics/article
Description
Abstract
Tissue engineering of an anterior cruciate
ligament (ACL) implant with ACL cells requires
detailed analysis of the tissue characteristics that should
be mimicked. Therefore, we studied the histological and
biochemical properties of rabbit derived ACLs in
comparison to other knee-associated tendons that are
used as autografts in men.
Rabbit derived ACLs and Musculus (M.) semimembranosus, M. semitendinosus tendons and patellar
ligaments were explanted from adult New Zealand white
rabbits and analyzed histologically for tissue
organization (e.g. cellularity, nuclear shapes, elastic
fibers), total collagen and sulfated glycosamino-glycan
(sGAG) contents. Gene expression analysis was
performed for the main extracellular matrix (ECM)
components type I collagen, decorin and the
glycoprotein tenomodulin.
The ACLs had an average dimension of
1.39x0.39x0.1 cm in situ. They were characterized by
high sGAG content in comparison to the other
tendons/ligaments, whereas the total collagen content
did not differ. ACLs possessed higher cellularity and
lower feret diameter of the cell nuclei compared with the
investigated rabbit-derived tendons. In ACLs long
elastic fibers were observed. Concerning the gene
expression level, lower transcription of tenomodulin was
detected in the ACL compared with the other tendons,
without significant difference in the decorin gene
expression. The M. semitendinosus tendon had a
significantly higher type I collagen expression than the
ACL and the other investigated tendons.
This phenotypical characterization of the lapine
ACL presented in this study provides some key
standards to evaluate tissue engineered ACL constructs
to be tested in the rabbit model.
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Citation
Histology and Histopathology, Vol.31, nº8, (2016)
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