Publication: Probing for protein-protein interactions during
cell migration: limitations and challenges
Authors
Chan, Jeremy Soon Kiat ; Teo, Zi Qiang ; Sng, Ming Keat ; Tan, Nguan Soon
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cellular migration is a fundamental
biological process occurring as early as embryogenesis
to the pathological state of cancer metastasis. Nearly all
cellular migrations involve an extracellular signal that is
transduced internally by members of a signalling
cascade. These signal transduction events are driven by
protein-protein interactions (PPIs) that coordinate
intracellular activities to enable a cell to migrate.
Understanding these PPIs will provide valuable insight
into how cellular migration can be modulated perhaps
towards a therapeutic end. Histologically, not many
techniques are available to demonstrate PPIs.
Contrasting agents only demonstrate the presence of a
particular protein, and perhaps its co-localisation with
another protein. Yet, co-localisation need not necessarily
indicate physical interaction between the two proteins. In
this review, we highlight four commonly used methods
that continue to expand our understanding of PPIs
underlying cell migration: co-immunoprecipitation,
bimolecular fluorescence complementation, proximity
ligation assay and surface plasmon resonance. The
methods discussed herein allow for the study of PPIs in a
wide variety of biological samples, including cell
lysates, live cells, fixed cells and tissues, enabling the
quantification of endogenous PPIs and exploration of the
nature of PPIs. We also include a rudimentary
framework for researchers to decide which experimental
method best suits their research goals.
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Citation
Histology and Histopathology, vol. 29, nº 8, (2014)
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