Publication:
Differentiation of human mesenchymal stromal cells cultured on collagen sponges for cartilage repair

Thumbnail Image
Files
Sanjurjo-Rodriguez-31-1221-1239-2016.pdf(17.66 MB)
Date
2016
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Sanjurjo Rodríguez, Clara ; Martínez Sánchez, Adela Helvia ; Hermida Gómez, Tamara ; Fuentes Boquete, Isaac ; Díaz Prado, Silvia ; Blanco, Francisco J.
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-11-754
item.page.type
info:eu-repo/semantics/article
Description
Abstract
y. Aim: The aim of this study was to evaluate proliferation and chondrogenic differentiation of human bone-marrow mesenchymal stromal cells (hBMSCs) cultured on collagen biomaterials. Materials and Methods: hBMSCs were seeded on five different collagen (Col) sponges: C1C2 (types I and II Col), C1C2HS (types I and II Col plus heparan sulphate (HS)), C1C2CHS (types I and II Col plus chondroitin sulphate (CHS)), C1-OLH3 (type I Col plus low molecular weight heparin) and C1CHS (type I Col plus CHS). The resulting constructs were analyzed by histological and immunohistochemical staining, molecular biology and electron microscopy. Col released into culture media was measured by a dye-binding method. Results: hBMSCs on biomaterials C1C2, C1C2HS and C1C2CHS had more capacity to attach, proliferate and synthesize Col II and proteoglycans in the extracellular matrix (ECM) than on C1-OLH3 and C1CHS. The presence of aggrecan was detected only at the gene level. Total Col liberated by the cells in the supernatants in all scaffold cultures was detected. The level of Col I in the ECM was lower in C1-OLH3 and that of Col II was highest in C1C2 and C1C2HS. Electron microscopy showed differently shaped cells, from rounded to flattened, in all constructs. Col fibers in bundles were observed in C1C2CHS by transmission electron microscopy. Conclusions: The results show that Col I and Col II (C1C2, C1C2HS and C1C2CHS) biomaterials allowed cell proliferation and chondrogenic-like differentiation of hBMSCs at an early stage. Constructs cultured on C1C2HS and C1C2CHS showed better cartilage-like phenotype than the other ones.
publication.page.subject
Tissue Engineering , Osteoarthritis , Mesenchymal Stromal Cells , Biomaterials , Chondrogenesis
Citation
URI
http://hdl.handle.net/10201/114045
item.page.embargo
Collections
Histology and histopathology Vol.31,nº 11 (2016)
Ir a Estadísticas