Publication: Apoptosis in peripheral neuroblastic
tumors. Immunohistochemical expression
of bcl-2 and p53 is related to DNA fragmentation
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Date
2007
Authors
Mejía, C. ; Navarro, S. ; Llombart-Bosch, A.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We examined 111 cases of neuroblastoma
(NB), searching for how NB relates to apoptotic control
and other prognostic factors. Immunohistochemistry
using avidin-biotin-peroxidase was carried out for bcl-2
and p53 proteins. Apoptosis was analyzed by in situ
detection of chromosomal breakdown. DNA ladders
were detected by electrophoresis and amplification of
MYCN was carried out by PCR and Southern blot.
Statistical analyses were performed with Pearson’s c2
and Kruskal-Wallis tests and Cox’s regression. We found
expression of bcl-2 protein mainly in cases of
neuroblastoma without differentiation and in stages 3
and 4. Expression of p53 protein showed a correlation
with bcl-2 and the apoptotic phenomenon; apoptosis was
found mainly in favorable cases. Multivariate analysis
showed bcl-2 protein expression to be the most
independent risk factor. The study of apoptosis could be
important for the design of therapies to treat
neuroblastoma.
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