Publication: MRL/MpJ mice show unique pathological features after experimental kidney injury
Authors
Shiozuru, Daichi ; Ichii, Osamu ; Kimura, Junpei ; Nakamura, Teppei ; Ali Elewa, Yaser Hosny ; Otsuka-Kanazawa, Saori ; Kon, Yasuhiro
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
10.14670/HH-11-662
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info:eu-repo/semantics/article
Description
Abstract
Clarification of the renal repair process is
crucial for developing novel therapeutic strategies for
kidney injury. MRL/MpJ mice have a unique repair
process characterized by low scar formation. The
pathological features of experimentally injured
MRL/MpJ and C57BL/6 mouse kidneys were compared
to examine the renal repair process.
The dilation and atrophy of renal tubules were
observed in folic acid (FA)-induced acute kidney injury
(AKI) in both strains, and the histopathological injury
scores and number of interleukin (IL)-1F6-positive
damaged distal tubules and kidney injury molecule 1
(KIM-1)-positive damaged proximal tubules drastically
increased 1 day after AKI induction. However, KIM-1-
positive tubules and the elevation of serum renal
function markers were significantly fewer and lower,
respectively, in MRL/MpJ mice at days 2 and 7 after
AKI. After traumatic kidney injury (TKI) via needle
puncture, severe tubular necrotic lesions in the punctured
area and fibrosis progressed in both strains. Indices for
fibrosis such as aniline blue-positive area, number of
alpha smooth muscle actin-positive myofibroblasts, and
messenger RNA expression levels of Tgfb1 and Mmp2
indicated lower fibrotic activity in MRL/MpJ kidneys.
Characteristically, only MRL/MpJ kidneys manifested
remarkable calcification around the punctured area
beginning 7 days after TKI.
The pathological features of injured MRL/MpJ and
C57BL/6 kidneys differed, especially those of kidneys
with mild proximal tubular injuries after FA-induced
AKI. Lower fibrotic activity and increased calcification
after TKI were observed in MRL/MpJ kidneys. These
findings clarified the unique pathological characteristics
of MRL/MpJ mouse kidneys and contribute to
understanding of the renal repair process after kidney
injury
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