Por favor, use este identificador para citar o enlazar este ítem:
10.14670/HH-30.725
Twittear
Título: | Effects of N-acetylcysteine on human ovarian tissue preservation undergoing cryopreservation procedure |
Fecha de publicación: | 2015 |
Editorial: | F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, vol. 30, nº 6, (2015) |
ISSN: | 1699-5848 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Cryopreservation of human ovarian tissue Reactive radical species N-acetylcysteine Electron paramagnetic resonance spectroscopy Light microscopy Transmission electron microscopy Ki67 immunohistochemical Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). |
Resumen: | The aim of the study was to evaluate the effects of the antioxidant N-acetylcysteine (NAC), added in freezing/thawing solutions, on reactive oxygen species (RRS) levels and on ovarian tissue preservation after cryopreservation. Ovarian samples from 10 subjects suffering from cancer diseases were cryopreserved using the slow freezing/rapid thawing standard protocol without or with NAC supplementation. RRS levels produced during cryopreservation were monitored by electron paramagnetic resonance (EPR) spectroscopy. The preservation of fresh ovarian tissue (t0), thawed tissue (t1 and t1 NAC) and thawed tissue maintained at 4°C for 2 hrs (t2 and t2 NAC) was analysed by light microscopy, transmission electron microscopy, Ki67 immunohistochemical and TUNEL analysis. It was possible to design a maximum peak for RRS production at t1, which slightly decreased at t2. NAC reduced the extent of RRS levels in cryopreserved ovarian tissues if compared with non-supplemented ones, although not restoring RRS production to baseline values. Comparative analysis between the two cryopreservation protocols showed that a better preservation of morphological characteristics, proliferation index and DNA integrity of ovarian tissue was obtained using NAC and no differences between t1NAC and t2NAC were observed. The employment of NAC during cryopreservation procedure could be anuseful strategy for preserving the function of endogenous cellular systems. Nevertheless, further studies on the viability of thawed ovarian tissue are needed to support the feasibility of this approach in clinical settings. |
Autor/es principal/es: | Fabbri, Raffaella Sapone, Andrea Paolini, Moreno Vivarelli, Fabio Franchi, Paola Lucarini, Marco Pasquinelli, Gianandrea Vicenti, Rossella Macciocca, Maria Venturoli, Stefano Canistro, Donatella |
URI: | http://hdl.handle.net/10201/96142 |
DOI: | 10.14670/HH-30.725 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 11 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International |
Aparece en las colecciones: | Vol.30, nº6 (2015) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Fabbri-30-725-735-2015.pdf | 8,07 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons