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Title: Histochemical and ultrastructural study of skeletal muscle in patients with sepsis and multiple organ failure syndrome (MOFS)
Issue Date: 1998
Publisher: F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Citation: Histology and histopathology, Vol. 13, nº 1 (1998)
ISSN: 1699-5848
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina
Keywords: Sepsis
Skeletal muscle
Abstract: Muscle biopsies for histochemical and ultrastuctural analysis were obtained from seven critically ill patients admitted to the Intensive Care Unit of the "Domingo Luciani" Hospital, Caracas, Venezuela. The sample included two patients with sepsis of abdominal origin, and five that presented sepsis/MOFS, with renal, hepatic, and respiratory disturbances and muscular weakness. Sections were examined for myosin adenosine triphosphatase (ATPase) after pre-incubation with both acid buffer (pH 4.37 and 4.6) and alkaline buffer (pH 10.3), for reduced nicotinamide dinucleotide diaphorase (NADHd), and for a-glycerophosphate dehydrogenase (a-GPDH). Sections were stained with hematoxilin and eosin to look for pathological changes and examined with a transmission electron microscope. Skeletal muscle of patients in early stage of sepsis showed a normal aspect with light microscopy, but at the ultrastructural level some of the fibres showed atrophy and some capillaries looked altered . Patients with sepsis/MOFS exhibited an evident muscle disorder with oedema. infiltrate, atrophy and segmental necrosis. All fibre types showed decrease in diameter; specially fibre types IIA and lIB . Intramuscular capillaries were thickened and occluded , indexes of capillarity were slightly reduced, and fibre oxidative activity was decreased. At ultrastructural level fibres showed severe atrophy, contractile system disorganization and segmental necrosis. Capillaries were also altered and the mononuclear cell infiltrate was abundant and represented by macrophages. lymphocytes and mastocytes.
Primary author: Díaz, N. L.
Finol, H. J.
Torres, S. H.
Zambrano, C. I.
Adjounian, H.
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 8
Rights: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 International
Appears in Collections:Vol.13, nº 1 (1998)

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