Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/92551

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dc.contributor.authorDíaz, N. L.-
dc.contributor.authorFinol, H. J.-
dc.contributor.authorTorres, S. H.-
dc.contributor.authorZambrano, C. I.-
dc.contributor.authorAdjounian, H.-
dc.date.accessioned2020-05-22T15:26:09Z-
dc.date.available2020-05-22T15:26:09Z-
dc.date.issued1998-
dc.identifier.citationHistology and histopathology, Vol. 13, nº 1 (1998)es_ES
dc.identifier.issn1699-5848-
dc.identifier.issn0213-3911-
dc.identifier.urihttp://hdl.handle.net/10201/92551-
dc.description.abstractMuscle biopsies for histochemical and ultrastuctural analysis were obtained from seven critically ill patients admitted to the Intensive Care Unit of the "Domingo Luciani" Hospital, Caracas, Venezuela. The sample included two patients with sepsis of abdominal origin, and five that presented sepsis/MOFS, with renal, hepatic, and respiratory disturbances and muscular weakness. Sections were examined for myosin adenosine triphosphatase (ATPase) after pre-incubation with both acid buffer (pH 4.37 and 4.6) and alkaline buffer (pH 10.3), for reduced nicotinamide dinucleotide diaphorase (NADHd), and for a-glycerophosphate dehydrogenase (a-GPDH). Sections were stained with hematoxilin and eosin to look for pathological changes and examined with a transmission electron microscope. Skeletal muscle of patients in early stage of sepsis showed a normal aspect with light microscopy, but at the ultrastructural level some of the fibres showed atrophy and some capillaries looked altered . Patients with sepsis/MOFS exhibited an evident muscle disorder with oedema. infiltrate, atrophy and segmental necrosis. All fibre types showed decrease in diameter; specially fibre types IIA and lIB . Intramuscular capillaries were thickened and occluded , indexes of capillarity were slightly reduced, and fibre oxidative activity was decreased. At ultrastructural level fibres showed severe atrophy, contractile system disorganization and segmental necrosis. Capillaries were also altered and the mononuclear cell infiltrate was abundant and represented by macrophages. lymphocytes and mastocytes.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent8es_ES
dc.languageenges_ES
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSepsises_ES
dc.subjectMOFSes_ES
dc.subjectSkeletal musclees_ES
dc.subjectUltrastructurees_ES
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes_ES
dc.titleHistochemical and ultrastructural study of skeletal muscle in patients with sepsis and multiple organ failure syndrome (MOFS)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
Appears in Collections:Vol.13, nº 1 (1998)



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