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Título: The vertebrae of prematurely aging mice as a skeletal model of involutional osteoporosis
Fecha de publicación: 2013
Editorial: F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, vol. 28, nº 11 (2013)
ISSN: 1699-5848
0213-3911
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.7 - Patología de los órganos de la locomoción. Sistema locomotor y esquelético
Palabras clave: Premature aging
Osteoporosis
Resumen: Oxidative stress in bone increases with age, which leads to bone frailty and a high fracture risk. Animal models show that early changes in trabecular structure occur in age-related osteopenia. These models might be valuable to assess the contribution of oxidative stress in age-related bone loss. Premature aging mice (PAM) have previously been characterized as a model of premature immunological and neurological senescence. PAM long bones (mainly consisting of cortical bone) display features of aging bone. Thus, we aimed to evaluate the vertebrae, representing a unique poorly loaded type of trabecular bone in mice, in PAM and no PAM (NPAM) controls. PAM showed an anxious behaviour, based on physical activity evaluation. These mice had decreased bone mineral density (0.078 mg/cm2 in NPAM vs 0.070 g/cm2 in PAM; p<0.05); a decreased number of osteocytes per bone field (404±36 in NPAM vs 320±27 in PAM; p<0.01); and downregulation of various osteoblastic genes and low eroded surface/bone surface, 4.2±0.5 in NPAM vs 1.9±0.2 in PAM; p<0.01). This was associated with increased expression of oxidative stress markers, Foxo1 and GADD45, in PAM vertebrae. Mesenchymal progenitors in the bone marrow of PAM have a poor mineralization capacity (assessed by the number of mineralized nodules and suface), and showed a lower response to an osteogenic input - represented by parathormone-related protein-, compared to NPAM. Collectively, these results indicate that PAM vertebrae show osteopenia related to diminished bone formation and remodeling. Our findings further support the validity of PAM as a suitable model for involutional osteoporosis and its treatment.
Autor/es principal/es: Portal Núñez, Sergio
Cruces, Julia
Lozano, Daniel
Ardura, Juan Antonio
Villanueva-Peñacarrillo, María L.
Fuente, Mónica de la
Esbrit, Pedro
URI: http://hdl.handle.net/10201/61587
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 9
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol.28, nº11 (2013)

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