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Título: | Differential expression and localization of transient receptor potential vanilloid 1 in rabbit and human eyes |
Fecha de publicación: | 2013 |
Editorial: | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, vol. 28, nº 11 (2013) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología |
Palabras clave: | TRPV1 Calcium channel |
Resumen: | Introduction: The superfamily of transient receptor potential (TRP) cation channels is involved in nociception. Members of this family, such as the vanilloid receptor type 1 (TRPV1) channel, are activated by a wide range of stimuli including heat (>43°C), low pH (<6.5), hypoxia, and hypertonicity. Here we report TRPV1 expression in rabbit and human eyes. Material and methods: We analyzed the expression of TRPV1 mRNA by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and protein by immunohistochemistry in eyes of New Zealand White rabbits and humans. Results: In rabbit and human eyes, TRPV1 protein was present in all layers of the corneal epithelium, but only in the basal layer of the conjunctiva. It was also in the ciliary and lens epithelia of both species as well as in the secretory cells of the rabbit lacrimal gland. The retinal pigment epithelium was positive for this protein in both species. TRPV1 was also present in rabbit Müller cells, where it had a similar pattern of expression to vimentin intermediate filaments. Analysis by qRT-PCR showed that TRPV1 mRNA was found in all of the structures where the protein was present. The highest level was in the lens and the lowest in the retina. Conclusion: TRPV1 is expressed in cells that are particularly active in Ca2+ exchange as well as in cells with significant water transport activity. Because TRPV1 is a Ca2+ channel, it probably functions in the regulation of both water and Ca2+ movements in ocular tissues. |
Autor/es principal/es: | Martínez-García, M. Carmen Martínez, Tamara Pañeda, Covadonga Gallego, Patricia Jimenez, Ana I. Merayo, Jesus |
URI: | http://hdl.handle.net/10201/61500 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.28, nº11 (2013) |
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Martinez-Garcia-28-1507-1516-2013.pdf | 32 MB | Adobe PDF | Visualizar/Abrir |
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