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Título: Proteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertension
Fecha de publicación: 2013
Editorial: F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, vol. 28, nº 7 (2013)
ISSN: 1699-5848
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
Palabras clave: Proteomics
Hypobaric hypoxia
Resumen: Experimental pulmonary hypertension that develops in hypobaric hypoxia is characterized by structural remodeling of the lung. Proteomics - which may be the most powerful way to uncover unknown remodeling proteins involved in enhancing cardiovascular performance - was used to study 150 male Wistar rats housed for up to 21 days in a chamber at the equivalent of 5500 m altitude level. After 14 days’ exposure to hypobaric hypoxia, pulmonary arterial pressure (PAP) was significantly increased. In lung tissue, about 140 matching protein spots were found among 8 groups (divided according to their hypobaric period) by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) (pH4.5-pH6.5, 30 kDa-100 kDa). In hypobaric rats, three spots were increased two-fold or more (vs. control rats) in two-dimensional differential in-gel electrophoresis (2D-DIGE). The increased proteins were identified, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), as one isoform of heat shock protein 70 (HSP70) and two isoforms of protein disulfide isomerase associated 3. This result was confirmed by Western blotting analysis of 2D-PAGE. Conceivably, HSP70 and PDIA3 may play roles in modulating the lung structural remodeling that occurs due to pulmonary hypertension in hypobaric hypoxia.
Autor/es principal/es: Ohata, Yuichiro
Ogata, Sho
Nakanishi, Kuniaki
Kanazawa, Fumiko
Uenoyama, Maki
Hiroi, Sadayuki
Tominaga, Susumu
Toda, Tosifusa
Kawai, Toshiaki
URI: http://hdl.handle.net/10201/59620
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 10
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol.28, nº 7 (2013)

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