Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10201/59620

Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorOhata, Yuichiro-
dc.contributor.authorOgata, Sho-
dc.contributor.authorNakanishi, Kuniaki-
dc.contributor.authorKanazawa, Fumiko-
dc.contributor.authorUenoyama, Maki-
dc.contributor.authorHiroi, Sadayuki-
dc.contributor.authorTominaga, Susumu-
dc.contributor.authorToda, Tosifusa-
dc.contributor.authorKawai, Toshiaki-
dc.identifier.citationHistology and Histopathology, vol. 28, nº 7 (2013)es
dc.description.abstractExperimental pulmonary hypertension that develops in hypobaric hypoxia is characterized by structural remodeling of the lung. Proteomics - which may be the most powerful way to uncover unknown remodeling proteins involved in enhancing cardiovascular performance - was used to study 150 male Wistar rats housed for up to 21 days in a chamber at the equivalent of 5500 m altitude level. After 14 days’ exposure to hypobaric hypoxia, pulmonary arterial pressure (PAP) was significantly increased. In lung tissue, about 140 matching protein spots were found among 8 groups (divided according to their hypobaric period) by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) (pH4.5-pH6.5, 30 kDa-100 kDa). In hypobaric rats, three spots were increased two-fold or more (vs. control rats) in two-dimensional differential in-gel electrophoresis (2D-DIGE). The increased proteins were identified, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), as one isoform of heat shock protein 70 (HSP70) and two isoforms of protein disulfide isomerase associated 3. This result was confirmed by Western blotting analysis of 2D-PAGE. Conceivably, HSP70 and PDIA3 may play roles in modulating the lung structural remodeling that occurs due to pulmonary hypertension in hypobaric hypoxia.es
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.subjectHypobaric hypoxiaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleProteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertensiones
Aparece en las colecciones:Vol.28, nº 7 (2013)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
Ohata-28-893-902-2013.pdf4,73 MBAdobe PDFVista previa

Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons