Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10201/59620
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Ohata, Yuichiro | - |
dc.contributor.author | Ogata, Sho | - |
dc.contributor.author | Nakanishi, Kuniaki | - |
dc.contributor.author | Kanazawa, Fumiko | - |
dc.contributor.author | Uenoyama, Maki | - |
dc.contributor.author | Hiroi, Sadayuki | - |
dc.contributor.author | Tominaga, Susumu | - |
dc.contributor.author | Toda, Tosifusa | - |
dc.contributor.author | Kawai, Toshiaki | - |
dc.date.accessioned | 2018-06-25T16:21:37Z | - |
dc.date.available | 2018-06-25T16:21:37Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Histology and Histopathology, vol. 28, nº 7 (2013) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/59620 | - |
dc.description.abstract | Experimental pulmonary hypertension that develops in hypobaric hypoxia is characterized by structural remodeling of the lung. Proteomics - which may be the most powerful way to uncover unknown remodeling proteins involved in enhancing cardiovascular performance - was used to study 150 male Wistar rats housed for up to 21 days in a chamber at the equivalent of 5500 m altitude level. After 14 days’ exposure to hypobaric hypoxia, pulmonary arterial pressure (PAP) was significantly increased. In lung tissue, about 140 matching protein spots were found among 8 groups (divided according to their hypobaric period) by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) (pH4.5-pH6.5, 30 kDa-100 kDa). In hypobaric rats, three spots were increased two-fold or more (vs. control rats) in two-dimensional differential in-gel electrophoresis (2D-DIGE). The increased proteins were identified, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), as one isoform of heat shock protein 70 (HSP70) and two isoforms of protein disulfide isomerase associated 3. This result was confirmed by Western blotting analysis of 2D-PAGE. Conceivably, HSP70 and PDIA3 may play roles in modulating the lung structural remodeling that occurs due to pulmonary hypertension in hypobaric hypoxia. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Proteomics | es |
dc.subject | Hypobaric hypoxia | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología | es |
dc.title | Proteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertension | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.28, nº 7 (2013) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Ohata-28-893-902-2013.pdf | 4,73 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons