Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/52405

Title: Local identification of porcine haptoglobin in salivary gland and diaphragmatic muscle tissues
Issue Date: 2012
Publisher: F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
Citation: Histology and histopathology, Vol. 27, nº 2 (2012)
ISSN: 1699-5848
0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos
Keywords: Pig
Immunohistochemistry
Abstract: In order to clarify the origin of the haptoglobin (Hp) quantified in saliva and meat juice samples, the extrahepatic localization of Hp in salivary gland and in diaphragmatic muscle, as part of the systemic acute phase response in pigs, was studied by immunohistochemistry. For this purpose a specific monoclonal antibody (mAb) produced by immunising mice with purified porcine Hp was used. Reactivity of the mAb was assessed by direct ELISA and by western blot, which showed the ability and specificity of the mAb to identify porcine haptoglobin as a purified antigen or in porcine serum in a native or denatured but non-reduced state. Five healthy and five diseased pigs were sampled at slaughter for serum and tissue procurement. Hepatic immunohistochemical analysis was used as control of the acute phase reaction status. In the liver, cell immunostaining revealed a perinuclear, cytoplasmic localization of Hp within hepatocytes, following mainly a periacinar pattern. Extrahepatic immunohistochemical analysis revealed positive cells in the glandular acini and duct epithelial cells of the salivary gland and intrasarcoplasmic immunolabelling of random diaphragmatic myofibers. A possible role of both salivary gland and diaphragmatic muscle on local Hp production could be postulated based on the present immunohistochemical study, which supports the concept that other cells besides hepatocytes may have the potential to produce Hp in the pig
Primary author: Gutiérrez Montes, Ana María
Yelamos, J.
Pallarés Martínez, Francisco José
Gómez Laguna, J.
Cerón Madrigal, José Joaquín
URI: http://hdl.handle.net/10201/52405
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 10
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.27, nº 2 (2012)

Files in This Item:
File Description SizeFormat 
Gutierrez-27-187-196-2012.pdf8,19 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons