Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/49356

Title: ß-Lapachone accelerates the recovery of burn-wound skin
Issue Date: 2011
Publisher: F. Hernández y J.F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología.
ISSN: 1699-5848
0213-3911
Related subjects: 576 - Biología celular y subcelular. Citología
Keywords: ß-lapachone
Wound healing
Abstract: ß-lapachone is a quinone of lapachol extracted from the bark of lapacho tree. Recent findings demonstrated that punched skin wounds of mice healed faster with ß-lapachone treatment. The present study investigates the effects of ß-lapachone on burn-wound skin of C57BL/6 mice injured by a 100°C iron stick. Our results indicated that wounds treated with ß-lapachone recovered faster than those treated with control ointment containing no ß-lapachone. On the third day after burning, the area of ß-lapachone treated-wound was 30% smaller than wound treated with control ointment. H&E and immunohistochemistry staining showed that burn-wound skin treated with ointment containing ß-lapachone healed faster in its epidermis, dermis, and underlying connective tissues with more macrophages appeared than those treated with control ointment alone. RAW264.7 cell, a macrophage-like cell line derived from BALB/C mice, was used as a model for scrutinizing the effect of ß-lapachone on macrophages. We found that the proliferation and the secretion of EGF and VEGF by macrophages were higher in cultures treated with ß-lapachone and that ß-lapachone can also increase the release of EGF with TNF-α pretreatment. We conclude that ß-lapachone plays an important role in accelerating burn wound healing, and that ß-lapachone not only can raise the proliferation of macrophages but also increase the release of VEGF from macrophages.
Primary author: Fu, Shih-Chen
Chau, Yat-Pang
Lu, Kuo-Shyan
Kung, Hsiu-Ni
Published in: Histology and histopathology, Vol. 26, nº7 (2011)
URI: http://hdl.handle.net/10201/49356
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 10
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.26, nº7 (2011)

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