Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/49322

Title: Immunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological condition
Issue Date: 2011
Publisher: F. Hernández y Juan F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología
ISSN: 1699-5848
0213-3911
Related subjects: 636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos
Keywords: Epidermis
Immunohistochemistry
Abstract: Epidermal homeostasis is maintained by both epithelial proliferation in the stratum basale (SB) and the apoptosis of epithelial cells under physiological conditions. In this study, the induction and regulation mechanisms of epidermal apoptosis were immunohistochemically investigated in the epidermis from Wistar rat’s palm and foot pad by using several apoptotic related proteins under a physiological condition. The results showed that Fas and Fas-L were expressed in cellular membranes of the stratum spinosum (SS), whereas TNF-R1 did not show any membranous expression in any epidermal layers. TNF-α was not observed in the epidermis. Caspase-10, cleaved caspase-3 and DNase-1 were found in the epithelial cytoplasms from the SS to stratum granulosum (SG), whereas caspase-8 was not detected in the epidermis. XIAP and Bak were found in the cytoplasm from the SS to SG, and the intensity of Bak-positivity was stronger in the SG than the SS, whereas Bid, Apaf-1 and cleaved caspase-9 were restricted in the SG. Homogenous cytoplasmic immunoreactivity of Bcl-2 was found in the SB and the intensity was gradually decreased from the SB to the SG. The granular-cytoplasmic immunopositivity of cytochrome C gradually altered into homogenous cytoplasmic expression in the upper half of the SG. Single-stranded DNA was rarely detected in the upper portion of the SG. These results suggest that epidermal apoptosis is induced by the interaction between Fas and Fas-L and the activation of caspase-10, and might initially proceed through a mitochondrialindependent pathway, and that a mitochondrialdependent pathway finally accelerated under physiological conditions.
Primary author: Udayanga, K.G.S
Miyata, H.
Yokoo, Y.
Qi, W.M.
Takahara, E.
Mantani, Y.
Yokoyama, T.
Hoshi, N.
Kitagawa, Hiroshi
Published in: Histology and histopathology, Vol. 26, nº 7 (2011)
URI: http://hdl.handle.net/10201/49322
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 10
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.26, nº7 (2011)

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