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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Baetas-da-Cruz, Wagner | es |
dc.contributor.author | Alves, Lucinéia | es |
dc.contributor.author | Guimarães, Erick V. | - |
dc.contributor.author | Santos-Silva, Alessandra | - |
dc.contributor.author | Pessolani, Maria Cristina V. | - |
dc.contributor.author | Barbosa, Helene S. | - |
dc.contributor.author | Corte-Rea, Suzana | - |
dc.contributor.author | Cavalcante, Leny A. | - |
dc.date.accessioned | 2013-09-24T11:40:02Z | - |
dc.date.available | 2013-09-24T11:40:02Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/36052 | - |
dc.description.abstract | Complex carbohydrate structures are essential molecules of infectious microbes and host cells, and are involved in cell signaling associated with inflammatory and immune responses. The uptake of mannose-tailed glycans is usually carried out by macrophages, dendritic cells (DCs), and other professional phagocytes to trigger MHC class I- and MHC class II-restricted antigen presentation, and to promote T cell effector responses. Since Schwann cells (SCs) have been proposed as immunocompetent cells, we investigated whether a human cell line (ST88-14 cells) could bind mannosylated ligands in a specific manner. The saturation of uptake of mannosylated molecules by ST88-14 cells and the internalization and distribution pathway of these ligands were tested by cytometry and confocal plus electron microscopy, respectively. This uptake showed a dose-dependent increase, the saturation point being reached at high concentrations of mannosyl residues/240mM mannose. Merging of man/BSA-FITC and S100 labeling showed their partial, but, significant colocalization. Ultrastructural analysis of ST88-14 cells after incubation with HRP-colloidal gold, without or with subsequent chasing at 37°C, showed an initial location on the cell surface and temperature- and time-dependent internalization of the probe. Our findings suggest an efficient mannosylated ligand uptake system through putative lectin(s) that may be operational in inflammatory and immune responses. | es |
dc.format | application/pdf | es |
dc.format.extent | 6 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Pattern recognition receptors | es |
dc.subject | Mannose receptor | es |
dc.subject.other | 57 - Biología | es |
dc.title | Efficient uptake of mannosylated proteins by a human Schwann cell line | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.24, nº8 (2009) |
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Efficient uptake of mannosylated proteins by a human Schwann cell line.pdf | 4,01 MB | Adobe PDF | Visualizar/Abrir |
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