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Título: | Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar |
Fecha de publicación: | 2008 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 616.3 - Patología del aparato digestivo. Odontología |
Palabras clave: | Tooth germ Hybridization |
Resumen: | This study examined detailed in situ expression patterns and possible functional roles of phosphoglycerate kinase 1 (Pgk1) gene in the developing tooth germ of the mouse lower first molar. The strong expression of Pgk1 mRNA was seen in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 (E10.5) to E18.0. Western blotting analysis demonstrated that Pgk1 protein formed 84-kDa protein complex in these embryonic organs. The results of immunoprecipitation-western blotting also suggested this complex to be formed with glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Moreover, the immunofluorescence expression of those proteins was shown to overlap each other in the tooth germ at E15.0. A strong immunofluorescence expression of both Pgk1 and GAPDH also corresponded to the in situ expression of those mRNAs. These results suggested that Pgk1 plays some functional roles in the development of tooth germ and other embryonic organs by forming protein complex with GAPDH. |
Autor/es principal/es: | Honda, Jun-ya Kobayashi, Leyoshi Kiyoshima, Tamotsu Yamaza, Haruyoshi Xie, Ming Takahashi, Keiko Enoki, Norio Nagata, Kengo Nakashima, Akihiko Sakai, Hidetaka |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/29685 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.23, nº4 (2008) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar.pdf | 8,63 MB | Adobe PDF | Visualizar/Abrir |
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