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Title: Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar
Issue Date: 2008
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: 616.3 - Patología del aparato digestivo. Odontología
Keywords: Tooth germ
Abstract: This study examined detailed in situ expression patterns and possible functional roles of phosphoglycerate kinase 1 (Pgk1) gene in the developing tooth germ of the mouse lower first molar. The strong expression of Pgk1 mRNA was seen in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 (E10.5) to E18.0. Western blotting analysis demonstrated that Pgk1 protein formed 84-kDa protein complex in these embryonic organs. The results of immunoprecipitation-western blotting also suggested this complex to be formed with glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Moreover, the immunofluorescence expression of those proteins was shown to overlap each other in the tooth germ at E15.0. A strong immunofluorescence expression of both Pgk1 and GAPDH also corresponded to the in situ expression of those mRNAs. These results suggested that Pgk1 plays some functional roles in the development of tooth germ and other embryonic organs by forming protein complex with GAPDH.
Primary author: Honda, Jun-ya
Kobayashi, Leyoshi
Kiyoshima, Tamotsu
Yamaza, Haruyoshi
Xie, Ming
Takahashi, Keiko
Enoki, Norio
Nagata, Kengo
Nakashima, Akihiko
Sakai, Hidetaka
Published in: Histology and histopathology
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 10
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.23, nº4 (2008)

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