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Título: | Nuclear localization of TRK-A in liver cells |
Fecha de publicación: | 2008 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 61 - Medicina |
Palabras clave: | Liver fibrosis Nerve growth factor |
Resumen: | The liver represents a site of expression of neurotrophins and their receptors. We have characterized the expression and intracellular localization of the nerve growth factor (NGF) receptor, Trk-A, in liver cells in vivo and in vitro. In both normal and fibrotic liver tissue, Trk-A immunostaining was present in different cell types, including parenchymal cells and cells of the inflammatory infiltrate. In hepatocytes and activated stellate cells (HSC), Trk-A showed a predominant nuclear localization, both in the presence and absence of injury. In cultured HSC, Trk-A was found to be functional, because exposure of the cells to recombinant NGF resulted in stimulation of cell migration and activation of intracellular signaling pathways, including Ras-ERK and PI3K/Akt. Remarkably, in cultured HSC, Trk-A staining was found constitutively in the nucleus. In these cells, Trk-A could be stained only by antibodies directed against the intracellular domain but not by those recognizing the extracellular portion of Trk-A suggesting that the intracellular portion of the receptor is the major determinant of nuclear Trk-A staining. In contrast to HSC, freshly isolated hepatocytes did not show any nuclear localization of the intracellular portion of Trk-A. In pheocromocytoma cells, nuclear staining for Trk-A was not present in conditions of serum deprivation, but could be induced by exposure to NGF or to a mixture of soluble mediators. We conclude that nuclear localization of the intracellular domain of Trk-A is observed constitutively in liver cells such as HSC, while in other cell types it could be induced in response to soluble factors. |
Autor/es principal/es: | Bonacchi, Andrea Taddei, María Leticia Petrai, LLaria Efsen, Eva DeFranco, Raffaella Nosi, Daniele Torcia, María Rosini, Paolo Formigli, Lucía Rombouts, Krista Zecchi, Sandra Milani, Stefano Pinzani, Massimo Laffi, Giacomo Marra, Fabio |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/29678 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 14 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.23, nº3 (2008) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Nuclear localization of TRK-A in liver cells.pdf | 5,79 MB | Adobe PDF | Visualizar/Abrir |
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