Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10201/29678
![](/digitum/image/email_logo.png)
![](/digitum/image/logo-facebook.png)
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Bonacchi, Andrea | es |
dc.contributor.author | Taddei, María Leticia | es |
dc.contributor.author | Petrai, LLaria | - |
dc.contributor.author | Efsen, Eva | - |
dc.contributor.author | DeFranco, Raffaella | - |
dc.contributor.author | Nosi, Daniele | - |
dc.contributor.author | Torcia, María | - |
dc.contributor.author | Rosini, Paolo | - |
dc.contributor.author | Formigli, Lucía | - |
dc.contributor.author | Rombouts, Krista | - |
dc.contributor.author | Zecchi, Sandra | - |
dc.contributor.author | Milani, Stefano | - |
dc.contributor.author | Pinzani, Massimo | - |
dc.contributor.author | Laffi, Giacomo | - |
dc.contributor.author | Marra, Fabio | - |
dc.date.accessioned | 2013-01-18T12:21:28Z | - |
dc.date.available | 2013-01-18T12:21:28Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/29678 | - |
dc.description.abstract | The liver represents a site of expression of neurotrophins and their receptors. We have characterized the expression and intracellular localization of the nerve growth factor (NGF) receptor, Trk-A, in liver cells in vivo and in vitro. In both normal and fibrotic liver tissue, Trk-A immunostaining was present in different cell types, including parenchymal cells and cells of the inflammatory infiltrate. In hepatocytes and activated stellate cells (HSC), Trk-A showed a predominant nuclear localization, both in the presence and absence of injury. In cultured HSC, Trk-A was found to be functional, because exposure of the cells to recombinant NGF resulted in stimulation of cell migration and activation of intracellular signaling pathways, including Ras-ERK and PI3K/Akt. Remarkably, in cultured HSC, Trk-A staining was found constitutively in the nucleus. In these cells, Trk-A could be stained only by antibodies directed against the intracellular domain but not by those recognizing the extracellular portion of Trk-A suggesting that the intracellular portion of the receptor is the major determinant of nuclear Trk-A staining. In contrast to HSC, freshly isolated hepatocytes did not show any nuclear localization of the intracellular portion of Trk-A. In pheocromocytoma cells, nuclear staining for Trk-A was not present in conditions of serum deprivation, but could be induced by exposure to NGF or to a mixture of soluble mediators. We conclude that nuclear localization of the intracellular domain of Trk-A is observed constitutively in liver cells such as HSC, while in other cell types it could be induced in response to soluble factors. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Liver fibrosis | es |
dc.subject | Nerve growth factor | es |
dc.subject.other | 61 - Medicina | es |
dc.title | Nuclear localization of TRK-A in liver cells | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.23, nº3 (2008) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Nuclear localization of TRK-A in liver cells.pdf | 5,79 MB | Adobe PDF | ![]() Visualizar/Abrir |
Los ítems de Digitum están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.