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Título: | Increased expression of 5-lipoxygenase is common in clear cell renal cell carcinoma |
Fecha de publicación: | 2007 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 616.6 - Patología del sistema genitourinario |
Palabras clave: | Renal cell carcinoma Tumor progression |
Resumen: | The clinical behaviour of Clear Cell Renal Cell Carcinoma (CC-RCC) is often unpredictable. To fully understand the signaling pathways involved in CCRCC development, we examined whether the 5- Lipoxygenase (5-LO), which catalyzes the biosynthesis of proinflammatory leukotrienes, is involved in renal tumorigenesis. By analyzing 46 snap-frozen primary renal cell carcinomas and their corresponding normal renal cortex biopsies, 5-LO protein levels were found to be significantly increased in the majority of CC-RCCs (P<0.001). Quantitative 5-LO mRNA expression analysis revealed up to 3-fold increased expression in the tumor tissues. There was no association between 5-LO and gender, grade or vein invasion. In contrast, increased 5-LO protein and mRNA correlated with large tumor size (>4 cm) and age of patients (P<0.001). 5-LO was frequently overexpressed in von Hippel-Lindau protein (pVHL)–reduced tumors and in Vascular Endothelial Growth Factor (VEGF)-positive tumors, which represent two frequent alterations in CC-RCC. Cell culture experiments demonstrated that VEGF expression was strongly inducible by 5-LO metabolites in RCC cell lines. The loss of pVHL expression led to high basal 5- LO and VEGF expression, which were markedly reduced by transfection with 5-LO small interfering RNA (siRNA). These results suggest that 5-LO upregulation is an important step in renal cancer progression. |
Autor/es principal/es: | Faronato, M. Muzzonigro, G. Milanese, G. Menna, C. Bonfigli, A.R. Catalano, A. Procopio, A. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/27642 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.22, nº10 (2007) |
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Increased expression of 5lipoxygenase is common in clear cell renal cell carcinoma.pdf | 4,36 MB | Adobe PDF | Visualizar/Abrir |
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