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Título: | Microarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation |
Fecha de publicación: | 2007 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 61 - Medicina |
Palabras clave: | Pneumocytes Pulmonary hypoplasia |
Resumen: | Fetal breathing-like movements (FBMs) are important in normal lung growth and pneumocyte differentiation. In amyogenic mouse embryos (designated as Myf5-/-:MyoD-/-, entirely lacking skeletal musculature and FBMs), type II pneumocytes fail to differentiate into type I pneumocytes, the cells responsible for gas exchange, and the fetuses die from asphyxia at birth. Using oligonucleotide microarrays, we compared gene expression in the lungs of Myf5-/- :MyoD-/- embryos to that in normal lungs at term. Nine genes were found to be up-regulated and 54 downregulated at least 2-fold in the lungs of double-mutant embryos. Since many down-regulated genes are involved in lymphocyte function, immunohistochemistry was employed to study T- and B-cell maturity in the thymus and spleen. Our findings of normal lymphocyte maturity implied that the down-regulation was specific to the double-mutant lung phenotype and not to its immune system. Immunostaining also revealed altered distribution of transcription and growth factors (SATB1, c-Myb, CTGF) from down-regulated genes whose knockouts are now known to undergo embryonic or neonatal death secondary to respiratory failure. Together, it appears that microarray analysis has identified a profile of genes potentially involved in pneumocyte differentiation and therefore in the mechanisms that may be implicated in the mechanochemical signal transduction pathways underlying FBMs-dependent pulmonary hypoplasia. |
Autor/es principal/es: | Baguma-Nibasheka, M. Angka, H.E. Inanlou, M.R. Kablar, B. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/27580 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 13 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.22, nº 5 (2007) |
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Microarray analysis of Myf5MyoD hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation.pdf | 3,1 MB | Adobe PDF | Visualizar/Abrir |
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