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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Baguma-Nibasheka, M. | es |
dc.contributor.author | Angka, H.E. | es |
dc.contributor.author | Inanlou, M.R. | - |
dc.contributor.author | Kablar, B. | - |
dc.date.accessioned | 2012-05-21T12:09:29Z | - |
dc.date.available | 2012-05-21T12:09:29Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/27580 | - |
dc.description.abstract | Fetal breathing-like movements (FBMs) are important in normal lung growth and pneumocyte differentiation. In amyogenic mouse embryos (designated as Myf5-/-:MyoD-/-, entirely lacking skeletal musculature and FBMs), type II pneumocytes fail to differentiate into type I pneumocytes, the cells responsible for gas exchange, and the fetuses die from asphyxia at birth. Using oligonucleotide microarrays, we compared gene expression in the lungs of Myf5-/- :MyoD-/- embryos to that in normal lungs at term. Nine genes were found to be up-regulated and 54 downregulated at least 2-fold in the lungs of double-mutant embryos. Since many down-regulated genes are involved in lymphocyte function, immunohistochemistry was employed to study T- and B-cell maturity in the thymus and spleen. Our findings of normal lymphocyte maturity implied that the down-regulation was specific to the double-mutant lung phenotype and not to its immune system. Immunostaining also revealed altered distribution of transcription and growth factors (SATB1, c-Myb, CTGF) from down-regulated genes whose knockouts are now known to undergo embryonic or neonatal death secondary to respiratory failure. Together, it appears that microarray analysis has identified a profile of genes potentially involved in pneumocyte differentiation and therefore in the mechanisms that may be implicated in the mechanochemical signal transduction pathways underlying FBMs-dependent pulmonary hypoplasia. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Pneumocytes | es |
dc.subject | Pulmonary hypoplasia | es |
dc.subject.other | 61 - Medicina | es |
dc.title | Microarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.22, nº 5 (2007) |
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Microarray analysis of Myf5MyoD hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation.pdf | 3,1 MB | Adobe PDF | Visualizar/Abrir |
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