Pulmonary lymphoid lesions in an experimental model of collapsing glomerulopathy in rats

Abstract

The characterization of lung damage in an experimental model of collapsing glomerulopathy (CG) in rats is described. Methods: 12 rats were divided into two groups and injected intravenously (iv) with 1 mg/ saline in a final volume of 1 ml/ day in the tail vein for 5 days, with fractionated serum from control and CG subjects. Proteinuria was quantified, and the Glomerular filtration rate was calculated based on creatinine clearance (CC). Rats were sacrificed by perfusion fixation at day 5. Results: Rats injected with serum from CG patients developed proteinuria (p<0.001). A decrease in CC (0.68±0.19) in these rats was also observed. Glomerular tuft retraction and mesangial proliferation was observed in all rats receiving serum from the CG patients. Peribronchiolar infiltrate integrated mainly by lymphocytes, was identified in all CG rats. In some areas this infiltration disrupted the basement membrane and damaged the epithelium. No histopathological abnormalities in the kidney or lungs were found in rats receiving control serum. Conclusion: Patchy pulmonary lymphoid infiltrates were found in the CG model. Up to now there was no information about pulmonary lymphoid infiltration in CG patients. Besides fluid overload due to renal insufficiency or a nephrotic syndrome, other causes of pulmonary involvement in CG patients should be explored.