Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/21556

Title: Involution of seminiferous tubules in aged hamsters: an ultrastructural, immunohistochemical and quantitative morphological study
Issue Date: 2004
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina
Keywords: Aging
Immunohistochemistry
Abstract: In this study, we examined the age-related changes on morphometric parameters and ultrastructure of seminiferous tubules, and on the expression of extracellular matrix proteins in lamina propria of Syrian hamsters. A significant decrease in the percentage of normal tubules and an increase in the percentage of hypospermatogenic and arrested maturation tubules was observed with aging. Aged animals showed a decrease in tubular diameter, tubular lumen, seminiferous epithelium volume and total tubular volume. However, the total length of seminiferous tubules was significantly increased with aging. The most important ultrastructural changes with aging were the thickening of the lamina propria, the presence of diverse abnormalities in the spermiogenesis process, degeneration of germ cells, and vacuolization and flattening of Sertoli cells showing abundant lipofucsin droplets and residual bodies. Laminin immunoreactivity was found along the lamina propria of seminiferous tubules both in young and aged animals. Fibronectin immunoreactivity was found along the lamina propria and blood vessels. Both laminin and fibronectin total volume of immunostaining per testis was increased in aged hamsters. In conclusion, the agerelated changes in seminiferous tubules of hamster include: a decrease in tubular width and an increase in tubular length; widening of the lamina propria caused by a more extensive connective matrix between the peritubular cells and the basal membrane; and a strong disarrangement of the seminiferous epithelium, including germ cell degeneration and important alterations in both spermiogenesis and Sertoli cell structure.
Primary author: Morales, E.
Horn, R.
Pastor, L.M.
Santamaria, L.
Pallarés, J.
Zuasti, A.
Ferrer, C.
Canteras, M.
Published in: Histology and histopathology
URI: http://hdl.handle.net/10201/21556
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 11
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.19, nº 2 (2004)

Files in This Item:
File Description SizeFormat 
Involution of seminiferous tubules in aged.pdf3,06 MBAdobe PDFThumbnail
View/Open


Items in Digitum are protected by copyright, with all rights reserved, unless otherwise indicated.