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Title: Immunoreactivity of Lewis blood group and mucin peptide core antigens: Correlations with grade of dysplasia and malignant transformation in the colorectal adenoma-carcinoma sequence
Issue Date: 2002
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina
Keywords: Colon-Cáncer
Abstract: Previous studies on the immunoreactivity of various mucin peptide and carbohydrate antigens in neoplastic colorectal tissues led to at least in part contradictory results. Therefore, we investigated a series of 42 adenomas and 44 carcinomas applying monoclonal antibodies (mabs) directed against Lewis blood group antigens (sialyl-Lea, Lex, sialyl-Lex, Ley) as well as mucin peptide cores (MUC1, MUC2 and MUC5AC) by i m m u n o h i s t o c h e m i s t r y. A statistically significant positive correlation between the development of highgrade dysplasia in colorectal adenomas and the immunoreactivity of Le y and MUC1 epitopes was observed, whereas MUC2 exhibited a significant negative correlation. The reactivity of the other epitopes did not show an association with the progression of malignant transformation. Colorectal carcinomas were subdivided according to their histopathological subtype. The immunohistochemical staining resulted in a significantly stronger MUC2 reactivity of mucinous vs. tubular adenocarcinomas. Immunoreactivity of the MUC1-specific mab, which does not react with the fully glycosylated peptide core, showed a statistically nonsignificant inverse tendency, whereas all carbohydrate antigens displayed a strong expression in both tumor subtypes. Furthermore, correlations between mucin peptide and carbohydrate epitope labelling were evaluated. Progression of the adenoma-carcinoma sequence was accompanied by an increase of Ley as well as MUC1 antigen and an increase of all Lewis antigens compared to MUC2 immunoreactivity. On the other hand, mucinous carcinomas exhibited an inverse pattern. In conclusion, these results demonstrate that Ley a n d MUC1 immunoreactivity correlate with malignant transformation in the colorectum, whereas MUC2 represents a marker for low-grade dysplasia and the subtype of mucinous carcinomas.
Primary author: Baldus, S.E.
Hanisch, F.G.
Pütz, C.
Flucke, U.
Mönig, S.P.
Schneider, P.M.
Thiele, J.
Hölscher, A.H.
Dienes, H.P.
Published in: Histology and histopathology
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 8
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.17, nº 1 (2002)

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