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Title: In vitro effects of estradiol on pituitary GH-immunoreactive cells
Issue Date: 1997
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina
Keywords: Estradiol
Abstract: In order to determine whether estradiol modulates the proliferation and activity of somatotrophic cells in vitro, a study of GH-immunoreactive cells was carried out in pituitary monolayer cultures obtained from male adult rats treated with ~ o - ~esMtrad iol for 3 hours. Cellular activity was evaluated in a morphometric study of GH-immunoreactive cells. The proliferation rate was determined by double immunostaining for GH and PCNA (proliferating cellular nuclear antigen). The results were compared to those obtained from control dishes. Estradiol was seen to increase the cellular (p<0.05), nuclear (pc0.01) and cytoplasmic areas (pc0.05). Estradiol decreased the percentage of proliferating GH-immunroeactive cells (pc0.05) and the numerical density of somatotrophs (pc0.05) when al1 cells present in the dishes were considered. However, when only GH-immunoreactive cells were considered, estradiol increased the proliferation rate of these cells (pc0.05). Overall, our results suggest that, in vitro, estradiol stimulates the cellular activity and proliferation of GH-immunoreactive cells in the rat.
Primary author: Carretero, J.
Rubio, M.
Sanchez, F.
Pereña, J.
Estévez, F.I.
Vázquez, G.
Pérez, R.M.
Vazquez, R.
Published in: Histology and histopathology
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 6
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.12, nº 3 (1997)

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