Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10201/18515

Título: Differentiation of human lymphocytes into nuclear vlimata by meiosis. The cytotoxic effect of calcium-activated neutral proteinase inhibitor
Fecha de publicación: 1994
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina
Palabras clave: Lymphocyte
Resumen: Phytohaemagglutinin (PHA)-activated lymphocytes differentiated into nuclear vlimata (NVs) in vitro. Lymphocyte attachment was followed by formation and extrusion of cytoplasmic vesicles. nuclear elongation and fragmentation into NVs. NVs and cytoplasmic vesicles were detached and organized into large cell nodules in suspension. Immunocytochemistry showed that T-lymphocytes differentiated mainly to NVs while B-lymphocytes to buds. During differentiation there was a loss or gain of T-antigenicity by either mother or daughter cell. Cytogenetic analysis by chromosomal spreading and in situ techniques showed that NVs do carry chromosomes of hypodiploid or hypohaploid sets. NVs were the result of meiosis stimulated by PHA. A ly~nphocyticp opulation exhibited diploidies attributed to mitosis or symmetrical meiosis and hypodiploidies, hypohaploidies attributed to meiosis. A comparison between NVs and spermatozoa was provided. The inhibitor of calcium-activated neutral proteinase (CANP-I) was cytotoxic to NVs without interfering with NV production. A model was proposed for the action of CANP-I which is a promising agent against autoimmune diseases.
Autor/es principal/es: Logothetou-Rella, H.
Publicado en: Histology and histopathology
URI: http://hdl.handle.net/10201/18515
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 16
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol. 9, nº 3 (1994)

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