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Título: Practical applications of some antibodies labelling the human retina
Fecha de publicación: 1993
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina
Palabras clave: Human retina
Monoclonal antibody
Resumen: Eight enucleated eyes were promptly fixed in 10% formalin and processed for light microscopy. Employing the ABC technique, we used antibodies against neuron-specific enolase (NSE), synaptophysin (SY), neurofilament (NF), glial fibrillary acidic protein (GFAP), keratins, LEU-7 and S-100 protein. Using NSE we found immunoreactivity in all sensory layers except the outer segments of photoreceptors which were nonreactive. SY showed a striking immunoreactivity of the synaptic regions (outer plexiform and inner plexiform layers). NF labelled the axons of the ganglion cells. GFAP stained perivascular glial cells and Muller cells only in areas of peripheral microcystoid degeneration. Keratin was non-reactive in all layers. LEU-7 showed that all layers were moderately reactive while the Muller cell processes and the outer limiting membrane were strongly immunoreactive. S-100 protein stained the perivascular glial cells in the nerve fiber layer. We conclude that NSE and LEU-7 are helpful markers for most layers of the sensory retina; GFAP and LEU-7 clearly outline Miiller cells and GFAP immunoreacts with astrocytes; NF is specific for axons and SY labels vividly the synaptic regions.
Autor/es principal/es: Chevez, P.
Font, R.L.
Forma parte de: Histology and histopathology
URI: http://hdl.handle.net/10201/18421
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 6
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol. 8, nº 3 (1993)

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