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Título: | Gonadotrophin-releasing hormone immunoreactivity in the brain of the tropical freshwater fish, Pygocentrus notatus (Teleostei-Characidae) |
Fecha de publicación: | 1993 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Hypothalamus Immunohistochemistry |
Resumen: | The distribution of GnRH in the brain of the teleost Pygocentrus notatus was demonstrated with the avidin-biotin peroxidase immunocytochemical method using highly specific antibody against synthetic mammalian GnRH. Optimal immunoreaction was obtained using: 1) Bouin's fluid for fixation; 2) repeated incubation with primary antiserum; 3) the use of a detergent in the dilution buffer; 4) the high sensitivity of the avidin-biotin immunoperoxidase method with the cobalt intensification of 3-3'diaminobenzidine tetrahydrochloride; and 5) the use of primary antibody with high specificity. GnRH-immunoreactive (GnRH-ir) in cells andlor axons was observed in al1 main brain regions. In the forebrain, GnRH-ir was located in a network extending from the caudal part of the olfactory bulb to the telencephalon. GnRH-ir fibres were also observed in the optic tectum, cerebellum and hypothalamus. Two groups of neurona1 cell bodies were identified. One group was located in the antero-ventral telencephalon corresponding to the nucleus olfactoretinalis. The second group was found in the rostrodorsal hypothalamus. No GnRH-ir material was detected in the pituitary gland, thus confirming the results of previous studies on brain GnRH-ir distribution obtained by radioimmunoanalysis in this species. These results demonstrate a high degree of similarity between the GnRH systems of P. notatus and other teleost species. |
Autor/es principal/es: | Cáceres-Dittmar, G. Tapia, F.J. Guerrero, H. Y. Paiva, C. L. Marcano, D. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/18374 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 6 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol. 8, nº 1 (1993) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Gonadotrophinreleasing hormone immunoreactivity in the brain of the tropical freshwater fish Pygocentrus notatus TeleosteiCharacidae.pdf | 3,39 MB | Adobe PDF | Visualizar/Abrir |
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