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|Title:||Gonadotrophin-releasing hormone immunoreactivity in the brain of the tropical freshwater fish, Pygocentrus notatus (Teleostei-Characidae)|
|Publisher:||Murcia : F. Hernández|
|Related subjects:||CDU::6 - Ciencias aplicadas::61 - Medicina|
|Abstract:||The distribution of GnRH in the brain of the teleost Pygocentrus notatus was demonstrated with the avidin-biotin peroxidase immunocytochemical method using highly specific antibody against synthetic mammalian GnRH. Optimal immunoreaction was obtained using: 1) Bouin's fluid for fixation; 2) repeated incubation with primary antiserum; 3) the use of a detergent in the dilution buffer; 4) the high sensitivity of the avidin-biotin immunoperoxidase method with the cobalt intensification of 3-3'diaminobenzidine tetrahydrochloride; and 5) the use of primary antibody with high specificity. GnRH-immunoreactive (GnRH-ir) in cells andlor axons was observed in al1 main brain regions. In the forebrain, GnRH-ir was located in a network extending from the caudal part of the olfactory bulb to the telencephalon. GnRH-ir fibres were also observed in the optic tectum, cerebellum and hypothalamus. Two groups of neurona1 cell bodies were identified. One group was located in the antero-ventral telencephalon corresponding to the nucleus olfactoretinalis. The second group was found in the rostrodorsal hypothalamus. No GnRH-ir material was detected in the pituitary gland, thus confirming the results of previous studies on brain GnRH-ir distribution obtained by radioimmunoanalysis in this species. These results demonstrate a high degree of similarity between the GnRH systems of P. notatus and other teleost species.|
|Primary author:||Cáceres-Dittmar, G.|
Guerrero, H. Y.
Paiva, C. L.
|Published in:||Histology and histopathology|
|Number of pages / Extensions:||6|
|Appears in Collections:||Vol. 8, nº 1 (1993)|
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|Gonadotrophinreleasing hormone immunoreactivity in the brain of the tropical freshwater fish Pygocentrus notatus TeleosteiCharacidae.pdf||3,39 MB||Adobe PDF|
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