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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Cáceres-Dittmar, G. | es |
dc.contributor.author | Tapia, F.J. | es |
dc.contributor.author | Guerrero, H. Y. | - |
dc.contributor.author | Paiva, C. L. | - |
dc.contributor.author | Marcano, D. | - |
dc.date.accessioned | 2011-02-01T09:16:05Z | - |
dc.date.available | 2011-02-01T09:16:05Z | - |
dc.date.issued | 1993 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/18374 | - |
dc.description.abstract | The distribution of GnRH in the brain of the teleost Pygocentrus notatus was demonstrated with the avidin-biotin peroxidase immunocytochemical method using highly specific antibody against synthetic mammalian GnRH. Optimal immunoreaction was obtained using: 1) Bouin's fluid for fixation; 2) repeated incubation with primary antiserum; 3) the use of a detergent in the dilution buffer; 4) the high sensitivity of the avidin-biotin immunoperoxidase method with the cobalt intensification of 3-3'diaminobenzidine tetrahydrochloride; and 5) the use of primary antibody with high specificity. GnRH-immunoreactive (GnRH-ir) in cells andlor axons was observed in al1 main brain regions. In the forebrain, GnRH-ir was located in a network extending from the caudal part of the olfactory bulb to the telencephalon. GnRH-ir fibres were also observed in the optic tectum, cerebellum and hypothalamus. Two groups of neurona1 cell bodies were identified. One group was located in the antero-ventral telencephalon corresponding to the nucleus olfactoretinalis. The second group was found in the rostrodorsal hypothalamus. No GnRH-ir material was detected in the pituitary gland, thus confirming the results of previous studies on brain GnRH-ir distribution obtained by radioimmunoanalysis in this species. These results demonstrate a high degree of similarity between the GnRH systems of P. notatus and other teleost species. | es |
dc.format | application/pdf | es |
dc.format.extent | 6 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Hypothalamus | es |
dc.subject | Immunohistochemistry | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina | es |
dc.title | Gonadotrophin-releasing hormone immunoreactivity in the brain of the tropical freshwater fish, Pygocentrus notatus (Teleostei-Characidae) | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol. 8, nº 1 (1993) |
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Fichero | Descripción | Tamaño | Formato | |
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Gonadotrophinreleasing hormone immunoreactivity in the brain of the tropical freshwater fish Pygocentrus notatus TeleosteiCharacidae.pdf | 3,39 MB | Adobe PDF | Visualizar/Abrir |
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