Electron microscopic study of the occlusible tapetum lucidum of the southern fiddler ray (Trygonorhina fasciata)

Abstract

The choroidally located tapetum lucidum of the southern fiddler ray (Trygonorhina fasciata) has been examined by light and electron microscopy in both light- and dark-adaptation. In this species. the tapetum consists of a single layer of overlapping cells oriented at an angle of about 30" to the incoming light. These are situated immediately external to the choriocapillaris. These tapetal cells alternate with and are separated from one another by melanocytes which have an inner extension that curves and intervenes between the tapetal cells and the choriocapillaris. The tapetal cells and the melanocytes are flattened cells with their widest dimension facing the retina. Internally the tapetal cells display a peripherally-located, vesicular nucleus with most of the cell organelles in a paranuclear location. The bulk of the cell is packed with regularly-spaced crystals reported to be guanine. The size and spacing of these reflective crystals is commensurate with constructive interference. In light-adaptation the small melanosomes of the melanocytes are widely dispersed and fill the portion of the cell intervening between the tapetal cells and the incoming light. This effectively occludes the tapetum as light is unable to reach the reflective material. In dark-adaptation the melanosomes withdraw from this location, exposing the tapetum to light and allowing it to act as a reflective layer. The retinal epithelium overlying the tapetal area is totally unpigmented so as not to interfere with the passage of light.