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Título: Decalcification by perfusion. A new method for rapid softening of temporal bones
Fecha de publicación: 1991
Editorial: Murcia : F. Hernández
ISSN: 0213-3911
Materias relacionadas: CDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomía
Palabras clave: Decalcification
Perfusion
Resumen: We describe a new technique, decalcification by perfusion, for the softening of bony tissue. The blood circulatory system was perfused in 16 rats via a cannula through the left heart ventricle with a fixative followed by New DecalcR (an acidic demineralizer) for 30-240 minutes. Perfusion decalcification for 120 minutes softened al1 heads and middle ear specimens could be easily sampled and prepared for studies by both light and electron microscope. For comparison, a conventional immersion technique required 72 hours of decalcification to accomplish softening. The perfusion technique considerably reduced the time needed to decalcify the tissue and preserved the morphology better than did the immersion procedure.
Autor/es principal/es: Nilsson, Magnus
Hellström, Sten
Albiin, Nils
Publicado en: Histology and histopathology
URI: http://hdl.handle.net/10201/18244
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 6
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol. 6, nº 3 (1991)

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