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|Title:||The thoracic sympathetic neurons of the chick, normal development and the effects of nerve growth factor|
|Publisher:||Murcia : F. Hernández|
|Related subjects:||CDU::6 - Ciencias aplicadas::61 - Medicina::612 - Fisiología|
Nerve growth factor
|Abstract:||The generation and degeneration of sympathetic neurons in the third thoracic ganglion (segment 19) of the chick were studied between embryonic days (E) 7-18 using 3H-Thymidine autoradiography and routine cell counts. Cumulative radiolabelling experiments indicated that few sympathetic neurons were generated on E6-7. 10% of the sympathetic neurons were generated on E8 and a further 20% on E9. The final 70% of neurons completed the mitotic cycle between E10-12. Cell counts demonstrated that the neuronal population increased from 10,166 + 423 (mean 2 SEM) to 22,291 ? 767 between E8-10 and remained stable up to E14. The population subsequently declined by 37%, to 14,157 + 831, by E18. Pyknotic neurons were found at all stages of development, but were most apparent between E7-15. The effects of Nerve Cirowth Factor (NGF) on the number of both surviving and pyknotic neurons in the ganglion were also examined. E9 embryos treated with NGF from E5-8 showed a 57% increase in the number of sympathetic neurons. This increase therefore occurred prior to the decline in neuronal number and was not accompanied by a decrease in the number of visibly pyknotic neurons. It is therefore possible that early NGF treatment increases the number of sympathetic neurons through a mechanism other than the attenuation of cell death.|
|Primary author:||Smet, Peter|
Rush, Robert A.
|Published in:||Histology and histopathology|
|Number of pages / Extensions:||7|
|Appears in Collections:||Vol. 1, nº 4 (1986)|
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