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Título: | Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro |
Fecha de publicación: | 1986 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Mellitic acid (MA) Sodium fluoride (NaF) |
Resumen: | The aim of this studv was to develop a standardized image analysis method for localization and quantitative measurement of calcified structures of murine fetal tibiae cultured in vitro as a completion and verification of previous biochemical studies. The calcified structures of bone stained by von Kossa silver technique and the epiphyseal cartilages showing intensive metachromasia with toluidine-blue staining were converted with grey-value window programs and afterwards the areas of the selected structures were measured. The histomorphological investigations showed that the murine tibiae, incubated for a period of 6 days in a medium with addition of 5 mmol mellitic acid, showed both a significant reduction of calcium deposits and an increase of epiphyseal intercellular cartilage matrix. The tibiae incubated in a medium with addition of 0.5 mmol sodium fluoride significantly showed an increase of calcium deposits in the thickened lammellae of the cornpacta. These histomorphological results confirm previous biochemical studies. |
Autor/es principal/es: | Weischer, C.H. Krisinger, J. Karzel, K. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/17832 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 6 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol. 1, nº 3 (1986) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Effects of mellitic acid MA and sodium fluoride NaF on the histological appearance of murine fetal tibiae cultured in vitro.pdf | 281,44 kB | Adobe PDF | Visualizar/Abrir |
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