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10.1016/j.theriogenology.2024.02.024
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Barranco, Isabel | - |
dc.contributor.author | Spinaci, Marcella | - |
dc.contributor.author | Nesci, Salvatore | - |
dc.contributor.author | Mateo-Otero, Yentel | - |
dc.contributor.author | Baldassarro, Vito Antonio | - |
dc.contributor.author | Algieri, Cristina | - |
dc.contributor.author | Bucci, Diego | - |
dc.contributor.author | Roca, Jordi | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal | es |
dc.date.accessioned | 2024-09-05T09:49:57Z | - |
dc.date.available | 2024-09-05T09:49:57Z | - |
dc.date.issued | 2024-04-15 | - |
dc.identifier.citation | Theriogenology;219:167-179. | es |
dc.identifier.issn | 1879-3231 | - |
dc.identifier.uri | http://hdl.handle.net/10201/143619 | - |
dc.description.abstract | Porcine seminal plasma (SP) is loaded with a heterogeneous population of extracellular vesicles (sEVs) that modulate several reproductive-related processes. This study investigated the effect of two sEV subsets, small (S-sEVs) and large (L-sEVs), on porcine in vitro fertilization (IVF). The sEVs were isolated from nine SP pools (five ejaculates/pool) using a size-exclusion chromatography-based procedure and characterized for quantity (total protein), morphology (cryogenic electron microscopy), size distribution (dynamic light scattering), purity and EV-protein markers (flow cytometry; albumin, CD81, HSP90β). The characterization confirmed the existence of two subsets of high purity (low albumin content) sEVs that differed in size (S- and L-sEVs). In vitro fertilization was performed with in vitro matured oocytes and frozen-thawed spermatozoa and the IVF medium was supplemented during gamete coincubation (1 h at 38.5 °C, 5 % CO2 in a humidified atmosphere) with three different concentrations of each sEV subset: 0 (control, without sEVs), 0.1, and 0.2 mg/mL. The first experiment showed that sEVs, regardless of subset and concentration, decreased penetration rates and total IVF efficiency (P < 0.0001). In a subsequent experiment, it was shown that sEVs, regardless of subset and concentration, impaired the ability of spermatozoa to bind to the zona pellucida of oocytes (P < 0.0001). The following experiment showed that sEVs, regardless of the subset, bound to frozen-thawed sperm but not to in vitro matured oocytes, indicating that sEVs would affect sperm functionality but not oocyte functionality. The lack of effect on oocytes was confirmed by incubating sEVs with oocytes prior to IVF, achieving sperm-zona pellucida binding results similar to those of control. In the last experiment, conducted under IVF conditions, sperm functionality was analyzed in terms of tyrosine phosphorylation, acrosome integrity and metabolism. The sEVs, regardless of the subset, did not affect sperm tyrosine phosphorylation or acrosome integrity, but did influence sperm metabolism by decreasing sperm ATP production under capacitating conditions. In conclusion, this study demonstrated that the presence of sEVs on IVF medium impairs IVF outcomes, most likely by altering sperm metabolism. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.relation | The study was funded by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 891382; grants PID2020-113493RB-I00/AEI/10.13039/501100011033 (Ministry of Science and Innovation [MICIN] Madrid, Spain), PID2022-137738NA-I00 funded by MCIN/AEI/10.13039/501100011033/FEDER UE, RYC2021–034546-I funded by MCIN/AEI/10.13039/501100011033 and European Union NextGenerationEU/PRTR; and grant 21935/PI/22 from the Séneca Foundation (Murcia, Spain). The authors are grateful to AIM Ibérica (Topigs Norsvin Ibérica, Madrid, Spain) for the supply of porcine ejaculates and to the technicians of the Department of Veterinary Medical Sciences of University of Bologna (Italy) for their technical assistance. In particular, the authors would like to thank Mrs. Cinzia Cappannari for her precious technical assistance. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Extracellular vesicles; In vitro fertilization; Porcine; Seminal plasma; Sperm. | es |
dc.title | Seminal extracellular vesicles alter porcine in vitro fertilization outcome by modulating sperm metabolism | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | 10.1016/j.theriogenology.2024.02.024 | - |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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1-s2.0-S0093691X2400092X-main.pdf | 6,15 MB | Adobe PDF | ![]() Visualizar/Abrir |
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