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Título: | Small and large extracellular vesicles of porcine seminal plasma differ in lipid profile |
Fecha de publicación: | 8-jul-2024 |
Editorial: | MDPI |
Cita bibliográfica: | Int. J. Mol. Sci. 2024, 25(13), 7492 |
ISSN: | Print: 1661-6596 Electronic: 1422-0067 |
Palabras clave: | Extracellular vesicles Lipidomics Porcine Seminal plasma Subsets |
Resumen: | Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile of two subsets of differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal plasma by size-exclusion chromatography and characterized by an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for lipidomic analysis. A total of 157 lipid species from 14 lipid classes of 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, and sterols) were identified. Qualitative differences were limited to two cholesteryl ester species present only in S-sEVs. L-sEVs had higher levels of all quantified lipid classes due to their larger membrane surface area. The distribution pattern was different, especially for sphingomyelins (more in S-sEVs) and ceramides (more in L-sEVs). In conclusion, this study reveals differences in the lipidomic profile of two subsets of porcine sEVs, suggesting that they differ in biogenesis and functionality. |
Autor/es principal/es: | Martínez Diaz, Pablo Parra, Ana Sánchez López, Christian M. Casas, Josefina Lucas, Xiomara Marcilla, Antonio Roca, Jordi Barranco, Isabel |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.mdpi.com/1422-0067/25/13/7492 |
URI: | http://hdl.handle.net/10201/143567 |
DOI: | https://doi.org/10.3390/ijms25137492 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 18 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © 2024 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms25137492 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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ijms-25-07492-v2.pdf | Published version | 5,55 MB | Adobe PDF | Visualizar/Abrir |
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