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https://doi.org/10.2436/20.1501.01.197
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Título: | Analysis of validamycin as a potential antifungal compound against Candida albicans |
Fecha de publicación: | 12-dic-2013 |
Editorial: | Institut d’Estudis Catalans |
Cita bibliográfica: | International Microbiology, 2013, Vol. 16, N. 4, pp. 217-225 |
ISSN: | Print: 1139-6709 Electronic: 1618-1905 |
Palabras clave: | Candida albicans Rhizoctonia solani Validamycin A Amphotericin B Trehalose |
Resumen: | Validamycin A has been successfully applied in the fight against phytopathogenic fungi. Here, the putative antifungal effect of this pseudooligosaccharide against the prevalent human pathogen Candida albicans was examined. Validamycin A acts as a potent competitive inhibitor of the cell-wall-linked acid trehalase (Atc1p). The estimated MIC50 for the C. albicans parental strain CEY.1 was 500 mg/l. The addition of doses below MIC50 to exponentially growing CEY.1 cells caused a slight reduction in cell growth. A concentration of 1 mg/ml was required to achieve a significant degree of cell killing. The compound was stable as evidenced by the increased reduction of cell growth with increasing incubation time. A homozygous atc1delta/atc1delta mutant lacking functional Atc1p activity showed greater resistance to the drug. The antifungal power of validamycin A was limited compared with the drastic lethal action caused by exposure to amphotericin B. The endogenous content of trehalose rose significantly upon validamycin and amphotericin B addition. Neither serum-induced hypha formation nor the level of myceliation recorded in macroscopic colonies were affected by exposure to validamycin A. Our results suggest that, although validamycin A cannot be considered a clinically useful antifungal against C. albicans, its mechanism of action and antifungal properties provide the basis for designing new, clinically interesting, antifungal-related compounds. |
Autor/es principal/es: | Guirao Abad, José P. Sánchez Fresneda, Ruth Valentín, Eulogio Martínez Esparza, M. Argüelles, Juan Carlos |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://revistes.iec.cat/index.php/IM/article/view/83098 |
URI: | http://hdl.handle.net/10201/142810 |
DOI: | https://doi.org/10.2436/20.1501.01.197 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | © 2013 Sociedad Española de Microbiología. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in International Microbiology. To access the final edited and published work see https://doi.org/10.2436/20.1501.01.197 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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