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https://doi.org/10.1016/j.cbpb.2018.04.006
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Título: | New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance |
Fecha de publicación: | 23-abr-2018 |
Editorial: | Elsevier |
Cita bibliográfica: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Volumes 221–222, July 2018, Pages 44-49 |
ISSN: | Print: 1096-4959 Electronic: 1879-1107 |
Palabras clave: | Antioxidants Assay validation Hypoxia Mussels Novel biomarkers Oxidants Oxidative stress |
Resumen: | The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress. |
Autor/es principal/es: | Franco-Martínez, Lorena Romero, Diego Peres Rubio, Camila Tecles, Fernando Martínez-Subiela, Silvia Teles, Mariana Tvarijonaviciute, Asta |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Ciencias Sociosanitarias |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S1096495918300484?via%3Dihub |
URI: | http://hdl.handle.net/10201/140159 |
DOI: | https://doi.org/10.1016/j.cbpb.2018.04.006 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 6 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2018 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Comparative Biochemistry and Physiology, Part B. To access the final edited and published work see https://doi.org/10.1016/j.cbpb.2018.04.006 |
Aparece en las colecciones: | Artículos: Ciencias Sociosanitarias |
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