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https://doi.org/10.1016/j.dci.2023.105124
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Título: | Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass |
Fecha de publicación: | mar-2024 |
Editorial: | Elsevier |
Cita bibliográfica: | Developmental & Comparative Immunology Volume 152, March 2024, 105124 |
ISSN: | 0145-305X |
Materias relacionadas: | European sea bass |
Palabras clave: | Cytotoxic T lymphocytes (CTLs) Perforin Granzyme Nodavirus DLB-1 |
Resumen: | Cell-mediated cytotoxicity (CMC) is essential in eradicating virus-infected cells, involving CD8+ T lymphocytes (CTLs) and natural killer (NK) cells, through the activation of different pathways. This immune response is well-studied in mammals but scarcely in teleost fish. Our aim was to investigate the adaptive CMC using head-kidney (HK) cells from European sea bass infected at different times with nodavirus (NNV), as effector cells, and the European sea bass brain cell line (DLB-1) infected with different NNV genotypes, as target cells. Results showed low and unaltered innate cytotoxic activity through the infection time. However, adaptive CMC against RGNNV and SJNNV/RGNNV-infected target cells increased from 7 to 30 days post-infection, peaking at 15 days, demonstrating the specificity of the cytotoxic activity and suggesting the involvement of CTLs. At transcriptomic level, we observed up-regulation of genes related to T cell activation, perforin/granzyme and Fas/FasL effector pathways as well as apoptotic cell death. Further studies are necessary to understand the adaptive role of European sea bass CTLs in the elimination of NNV-infected cells. |
Autor/es principal/es: | Garcia Alvarez, M. A. Chaves Pozo, E. Cuesta Peñafiel, A. |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0145305X23012776?via%3Dihub |
URI: | http://hdl.handle.net/10201/139389 |
DOI: | https://doi.org/10.1016/j.dci.2023.105124 |
Tipo de documento: | info:eu-repo/semantics/article |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International |
Descripción: | © 2023. The authors. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc /4.0/ This document is the accepted version of a published work that appeared in final form in Developmental and Comparative Immunology |
Aparece en las colecciones: | Artículos: Biología Celular e Histología |
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